3KYS
Crystal structure of human YAP and TEAD complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97869 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.643, 110.503, 165.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.160 - 2.800 |
| R-factor | 0.2296 |
| Rwork | 0.228 |
| R-free | 0.26580 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.063 | 0.354 |
| Number of reflections | 20593 | 20593 |
| <I/σ(I)> | 44.3 | 3.91 |
| Completeness [%] | 97.9 | 83.6 |
| Redundancy | 13.8 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 0.1M sodium acetate, 2M sodium formate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
