3KYK
Crystal structure of li33 Igg1 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97961 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 90.632, 90.632, 215.041 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.340 - 3.200 |
| R-factor | 0.198 |
| Rwork | 0.194 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b2x |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.381 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.221 | 0.571 |
| Number of reflections | 9258 | |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.8 | 17.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 2M ammonium sulfate, 0.1M sodium acetate pH 3.5 and 0.1M TCEP, vapor diffusion, hanging drop, temperature 293K |
