3KXK
Crystal structure of SsGBP mutation variant G235P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-02 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.710, 71.239, 97.672 |
| Unit cell angles | 90.00, 96.81, 90.00 |
Refinement procedure
| Resolution | 38.819 - 2.350 |
| R-factor | 0.245 |
| Rwork | 0.244 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qtf |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.431 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
| Rmerge | 0.051 | 0.023 | 0.408 |
| Number of reflections | 31222 | ||
| <I/σ(I)> | 13.2 | ||
| Completeness [%] | 98.4 | 99.1 | 97.2 |
| Redundancy | 4 | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 12% (w/v) PEG 3350, 0.18M sodium thiocyanate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
