3KXI

crystal structure of SsGBP and GDP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Collection date	2009-03-16
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	67.322, 68.471, 92.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.227 - 2.650
R-factor	0.227
Rwork	0.224
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.828
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.740
High resolution limit [Å]	2.650	5.710	2.650
Rmerge	0.079	0.049	0.374
Number of reflections	11840
<I/σ(I)>	14.2
Completeness [%]	91.6	81.3	87.9
Redundancy	2.7	2.6	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	10% (w/v) PEG 3350, 0.18M sodium thiocyanate, VAPOR DIFFUSION, HANGING DROP, temperature 289K