3KXD
Crystal structure of the mthk rck in complex with cadmium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-05 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.022, 38.293, 96.634 |
| Unit cell angles | 90.00, 95.10, 90.00 |
Refinement procedure
| Resolution | 57.830 - 2.200 |
| R-factor | 0.1878 |
| Rwork | 0.184 |
| R-free | 0.25364 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2aej |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.877 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.830 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.039 |
| Number of reflections | 21805 |
| <I/σ(I)> | 10.8 |
| Completeness [%] | 99.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 318 | 1M NH4-FORMATE, 10% PEG 4000, 0.1M NA ACETATE, PH 4.7, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 318K |
