3KX9
Engineering a closed form of the Archaeoglobus fulgidus ferritin by site directed mutagenesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 123.589, 123.596, 350.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.954 - 2.101 |
| R-factor | 0.1855 |
| Rwork | 0.183 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s3q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.952 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.954 | 2.180 |
| High resolution limit [Å] | 2.101 | 2.100 |
| Rmerge | 0.074 | 0.434 |
| Number of reflections | 286181 | |
| <I/σ(I)> | 25.01 | |
| Completeness [%] | 92.0 | 91.4 |
| Redundancy | 8.3 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298.15 | 41.6mM sodium citrate dihydrate pH 5.6, 1.04 M 1,6-Hexanediol, 31.25% glycerol, 166.7 mM Na-L-tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
