3KWM
Crystal structure of ribose-5-isomerase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 182.360, 73.085, 75.303 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.680 - 2.320 |
| R-factor | 0.17252 |
| Rwork | 0.170 |
| R-free | 0.22110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3enq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.101 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.566 |
| Number of reflections | 1052731 | |
| <I/σ(I)> | 24.075 | 2.356 |
| Completeness [%] | 98.2 | 71.2 |
| Redundancy | 6.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2M Lithium Chloride, 0.1M Tris, 20% PEG 6000 , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
