3KWD
Inactive truncation of the beta-carboxysomal gamma-Carbonic Anhydrase, CcmM, form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91840 |
| Spacegroup name | H 3 |
| Unit cell lengths | 103.006, 103.006, 44.953 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.970 - 1.100 |
| R-factor | 0.13344 |
| Rwork | 0.133 |
| R-free | 0.14769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qre |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.383 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 70535 | |
| <I/σ(I)> | 24.1 | 2.66 |
| Completeness [%] | 97.8 | 77.2 |
| Redundancy | 3.25 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 14 mg/ml protein, 1.0 M ammonium sulphate, 0.1 M Bis-Tris, 1% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
