3KW8
Two-domain laccase from Streptomyces coelicolor at 2.3 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 176.723, 176.723, 176.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.560 - 2.290 |
| R-factor | 0.16193 |
| Rwork | 0.161 |
| R-free | 0.17817 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cg8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.483 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.290 | 2.300 |
| Rmerge | 0.114 | 0.605 |
| Number of reflections | 42751 | |
| <I/σ(I)> | 33.9 | 6.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.1 | 24.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 40 %(v/v) PEG 550 monomethyl ether, 0.1M NaCl, 0.1M glycine, 50mM potassium hexacyanoferrate , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
