3KVW
Crystal Structure of dual-specificity tyrosine phosphorylation regulated kinase 2 (DYRK2) in complex with an indirubin ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9802 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 84.280, 84.280, 149.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.810 - 2.280 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k2l |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.548 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.552 | 2.400 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.105 | 0.885 |
| Number of reflections | 25294 | |
| <I/σ(I)> | 12.2 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2M Na(Acetate), 0.1M cacodylate pH 6.5, 30% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
