3KVH
Crystal structure of human protein syndesmos (NUDT16-like protein)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 68.811, 60.278, 60.483 |
| Unit cell angles | 90.00, 118.64, 90.00 |
Refinement procedure
| Resolution | 27.010 - 1.700 |
| R-factor | 0.18522 |
| Rwork | 0.184 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cou |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.025 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.829 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.069 | 0.777 |
| Number of reflections | 23914 | |
| <I/σ(I)> | 8.8 | 1.9 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M hepes pH 7.5, 20%w/v jeffamine M-600, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
