3KVC
Crystal structure of bovine RPE65 at 1.9 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03324 |
| Spacegroup name | P 65 |
| Unit cell lengths | 176.356, 176.356, 86.715 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.14758 |
| Rwork | 0.146 |
| R-free | 0.17062 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3fsn |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.455 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.104 | 0.565 |
| Number of reflections | 119836 | |
| <I/σ(I)> | 17.5 | 2.26 |
| Completeness [%] | 99.0 | 92 |
| Redundancy | 4.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 30% PEG 200 AS PRECIPITANT , pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
