3KUZ

Crystal structure of the ubiquitin like domain of PLXNC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97927
Spacegroup name	P 43 21 2
Unit cell lengths	72.246, 72.246, 116.235
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.251
Rwork	0.250
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	superimposed ensemble of PDB entries 3h6n and 3ig3
RMSD bond length	0.015
RMSD bond angle	1.466
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.340
High resolution limit [Å]	2.300	6.180	2.300
Rmerge	0.187	0.057	0.963
Number of reflections	14277
<I/σ(I)>	4.1
Completeness [%]	99.6	98.4	98.3
Redundancy	9	8.5	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	2.0M ammonium sulfate, 0.2M sodium chloride, 0.1M HEPES, 1:100 w/w chymotrypsin, pH 7.5, vapor diffusion, sitting drop, temperature 291K