3KU7

Crystal structure of Helicobacter pylori MinE, a cell division topological specificity factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 64
Unit cell lengths	70.840, 70.840, 65.509
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.780 - 2.800
R-factor	0.268
Rwork	0.268
R-free	0.29800
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	4582
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100mM MES-NaOH (pH 6.5), 26% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100mM MES-NaOH (pH 6.5), 26% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K