3KU6
Crystal structure of a H2N2 influenza virus hemagglutinin, 226L/228G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97650 |
Spacegroup name | P 63 |
Unit cell lengths | 70.484, 70.484, 236.844 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.000 - 1.750 |
R-factor | 0.19253 |
Rwork | 0.191 |
R-free | 0.22355 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.493 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (1.3.3) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.071 | 0.330 |
Number of reflections | 66916 | |
<I/σ(I)> | 16.3 | 3.5 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 5.6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 295 | 30% PEG 3000, 0.1M Tris, pH 7.8, vapor diffusion, sitting drop, temperature 295K |