3KTW
Crystal structure of the SRP19/S-domain SRP RNA complex of Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.307, 79.338, 114.067 |
| Unit cell angles | 90.00, 101.99, 90.00 |
Refinement procedure
| Resolution | 64.680 - 3.200 |
| R-factor | 0.242 |
| Rwork | 0.240 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lng |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.619 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.700 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 20578 | |
| <I/σ(I)> | 13.6 | 3.3 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 100 mM Na cacodylate, 400 mM KCl, 10 mM CaCl2, 15% (w/v) PEG4000, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Na cacodylate | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 1 | PEG4000 | ||
| 5 | 1 | 2 | Na cacodylate | ||
| 6 | 1 | 2 | KCl | ||
| 7 | 1 | 2 | CaCl2 | ||
| 8 | 1 | 2 | PEG4000 |
