3KTF
Structure of the N-terminal BRCT domain of human microcephalin (MCPH1).
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.069, 39.274, 106.639 |
| Unit cell angles | 90.00, 91.52, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.139 |
| Rwork | 0.138 |
| R-free | 0.19500 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.046 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.550 |
| Rmerge | 0.059 | 0.397 |
| Number of reflections | 36909 | |
| <I/σ(I)> | 46.07 | 2.95 |
| Completeness [%] | 88.9 | 64.1 |
| Redundancy | 6.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 295 | 0.2M sodium succinate, 15% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
