3KT0

Crystal structure of S. cerevisiae tryptophanyl-tRNA synthetase.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-10
Detector	ADSC QUANTUM 4r
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	55.912, 55.912, 313.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.450 - 2.100
R-factor	0.208
Rwork	0.207
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o5t
RMSD bond length	0.008
RMSD bond angle	1.075
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.102	0.049	0.586
Number of reflections	29469
<I/σ(I)>	7.2
Completeness [%]	96.6	87.7	98.5
Redundancy	9.1	7.8	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1M MES buffer, 0.05M CsCl, 30% v/v Jeffamine M-600 reagent, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K