3KSK

Crystal Structure of single chain PvuII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Detector	MAR CCD 165 mm
Wavelength(s)	0.81280
Spacegroup name	P 42
Unit cell lengths	101.921, 101.921, 100.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	102.060 - 2.350
R-factor	0.219
Rwork	0.215
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	2.087
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.060	2.411
High resolution limit [Å]	2.350	2.350
Number of reflections	42525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	1.28-1.36 M ammonium sulfate, 5% MPD, 100 mM bis-Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K