3KRN
Crystal Structure of C. elegans cell-death-related nuclease 5(CRN-5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 102 |
| Detector technology | CCD |
| Collection date | 2008-11-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.954, 44.837, 65.507 |
| Unit cell angles | 90.00, 100.65, 90.00 |
Refinement procedure
| Resolution | 26.505 - 3.918 |
| R-factor | 0.3101 |
| Rwork | 0.306 |
| R-free | 0.35640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nn6 chain D |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.989 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.040 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.071 | 0.197 |
| Total number of observations | 9991 | |
| Number of reflections | 3235 | |
| <I/σ(I)> | 13.8 | 5.25 |
| Completeness [%] | 97.7 | 91.3 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M Tris-HCl, 0.2M NaCl, 25% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
