3KR3
Crystal structure of IGF-II antibody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95363 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.667, 106.921, 110.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.370 - 2.200 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.780 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.400 | 2.257 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 26600 | |
| Completeness [%] | 89.6 | 90.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.3 | 293 | 18.5% PEG 6000, 118mM CaCl2, 10% (v/v) PCB buffer (propionate-cacodylate-bis-tris propane buffer), pH 5.3, vapor diffusion, temperature 293K |
