3KQR
The structure of serum amyloid p component bound to phosphoethanolamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.860, 69.860, 102.270 |
| Unit cell angles | 90.00, 96.95, 90.00 |
Refinement procedure
| Resolution | 36.811 - 1.500 |
| R-factor | 0.142 |
| Rwork | 0.141 |
| R-free | 0.16100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.579 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.812 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.053 | 0.180 |
| Number of reflections | 208934 | |
| <I/σ(I)> | 16.8 | 4 |
| Completeness [%] | 98.7 | 97.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.06M TRIS-HCL pH8, 16% PEG 550MME, 0.01M CaCl2, 0.14M NaCl, 0.1% NaN3,14.2mg/ml Protein, 0.05M Ligand, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
