3KOW
Crystal Structure of ornithine 4,5 aminomutase backsoaked complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-07 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.180, 229.010, 123.920 |
Unit cell angles | 90.00, 102.76, 90.00 |
Refinement procedure
Resolution | 120.860 - 2.900 |
R-factor | 0.195 |
Rwork | 0.191 |
R-free | 0.27900 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.611 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 120.860 | 2.970 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.108 | 0.278 |
Number of reflections | 72733 | |
<I/σ(I)> | 8.3 | 3.4 |
Completeness [%] | 97.9 | 96.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 294 | A protein solution (8 mg/ml 4,5-OAM, 5 mM 2-mercaptoethanol, 10 mM Tris HCl pH 8.0, 2 mM PLP, and 2 mM AdoCbl) was mixed in a 1:1 ratio with precipitant solution [(0.1 M Tris HCl, pH 8.0, 0.2 M MgCl2, 25 % (wt/vol) polyethylene glycol 2000 mono-methylether] under red light at room temperature., VAPOR DIFFUSION, SITTING DROP, temperature 294K |