3KOL
Crystal structure of a glyoxalase/dioxygenase from Nostoc punctiforme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 50.382, 50.382, 138.026 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.095 | 0.357 |
| Number of reflections | 14757 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 54.8 | 55.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | Ammonium Sulfate; MES pH 6.5; Cobalt (II) Chloride hexahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
