3KO1
Cystal structure of thermosome from Acidianus tengchongensis strain S5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.90000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 223.674, 283.042, 160.750 |
Unit cell angles | 90.00, 133.90, 90.00 |
Refinement procedure
Resolution | 48.390 - 3.700 |
R-factor | 0.277 |
Rwork | 0.277 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q3r |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.900 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.117 | 0.476 |
Number of reflections | 76576 | |
<I/σ(I)> | 7.4 | 2.3 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris-Hcl, 1.15M Li2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |