3KND

TPX2:importin-alpha complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9814
Spacegroup name	P 21 21 21
Unit cell lengths	78.527, 89.317, 99.273
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.712 - 2.151
R-factor	0.1846
Rwork	0.182
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eyj
RMSD bond length	0.007
RMSD bond angle	0.993
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.850	2.320
High resolution limit [Å]	2.151	2.200
Rmerge	0.109	0.959
Number of reflections	36111
<I/σ(I)>	11.9	2.2
Completeness [%]	99.9	100
Redundancy	6.9	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	1.1-1.2 M ammonium sulphate, 100 mM Tris-Hcl, 10 mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K