3KMU
Crystal structure of the ILK/alpha-parvin core complex (apo)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97880, 0.97900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.380, 117.715, 47.344 |
| Unit cell angles | 90.00, 101.68, 90.00 |
Refinement procedure
| Resolution | 58.820 - 1.800 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.484 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.820 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 94370 | |
| <I/σ(I)> | 28.2 | 2.3 |
| Completeness [%] | 99.9 | 96.9 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 277 | 0.05 M Bis-Tris, 12% PEG 5000 MME, 5% 1-Propyl alcohol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
