3KMN
Crystal Structure of the Human Apo GST Pi C47S/Y108V Double Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.957 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.320, 89.430, 69.200 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 34.610 - 1.800 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.21000 |
| Structure solution method | Difference Fourier |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.352 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.260 |
| Number of reflections | 39585 | 6117 |
| <I/σ(I)> | 22.8 | 5.1 |
| Completeness [%] | 94.0 | 97.7 |
| Redundancy | 10.1 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 7.5mg/ml C47S/Y108V GST, 22% (w/v) PEG 8000, 100mM MES pH 6.0, 350mM Ca acetate, 10mM DTT, vapor diffusion, hanging drop, temperature 298K |
