3KLZ
Pentameric formate channel with formate bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-24 |
Detector | ADSC |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.779, 100.456, 192.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.240 - 2.500 |
R-factor | 0.175 |
Rwork | 0.173 |
R-free | 0.22300 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 62189 |
<I/σ(I)> | 14.6 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.02M MOPS, PH 6.8, 29% (W/V) PEG 550MME, 120MM SODIUM FORMATE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K |