3KLS
Structure of complement C5 in complex with SSL7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0379 |
| Spacegroup name | P 31 |
| Unit cell lengths | 143.875, 143.875, 241.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.047 - 3.600 |
| R-factor | 0.2009 |
| Rwork | 0.198 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3CU7 chain A residues 20-1510 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.658 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.047 | 3.800 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 64555 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | 277 | Reservoir contains 50mM MgAc2, 50 mM MES pH 6.2, mixed 1:1 with protein, VAPOR DIFFUSION, temperature 277K |
