3KL8
CaMKIINtide Inhibitor Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | .9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.410, 83.080, 145.130 |
| Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
| Resolution | 71.708 - 3.372 |
| R-factor | 0.234 |
| Rwork | 0.230 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.101 |
| Data reduction software | MOSFLM (3.3.9) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.100 | 3.600 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 21523 | |
| Completeness [%] | 98.3 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 20% 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, temperature 293K |
