3KKO
Crystal structure of M-Ras P40D/D41E/L51R in complex with GppNHp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-10 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.196, 192.248, 43.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.570 - 1.900 |
| R-factor | 0.18584 |
| Rwork | 0.184 |
| R-free | 0.22192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x1s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.198 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.570 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.099 | 0.282 |
| Number of reflections | 52129 | |
| <I/σ(I)> | 3.8 | 2.5 |
| Completeness [%] | 99.2 | 94.8 |
| Redundancy | 6.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | Ammonium sulfate, sodium acetate, PEG-4000, glycerol, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
