3KKE
Crystal structure of a LacI family transcriptional regulator from Mycobacterium smegmatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97958 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.599, 65.468, 116.034 |
Unit cell angles | 90.00, 90.72, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.24700 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.477 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.9) |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.367 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.083 | 0.371 |
Number of reflections | 49080 | |
<I/σ(I)> | 14.2 | 5 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 7.5 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 294 | 100mM sodium acetate pH 4.6, 30% PEG 4000, 200mM ammonium acetate, vapor diffusion, temperature 294K |