3KJY

Crystal structure of reduced HOMO SAPIENS CLIC3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-11-03
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	48.080, 48.380, 59.760
Unit cell angles	69.68, 80.89, 74.60

Refinement procedure

Resolution	30.260 - 1.950
R-factor	0.2003
Rwork	0.198
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fy7
RMSD bond length	0.007
RMSD bond angle	1.021
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.4_161))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.260	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.036	0.406
Number of reflections	32724
<I/σ(I)>	15.7	2.1
Completeness [%]	92.5	89.4
Redundancy	2.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Solution (15mg/ml protein in 20mm HEPES-NAOH, PH 7.5, 200mm NACl) plus 3 micro-l of reservoir solutionl (0.95M NH4SO4,0.225M LISO4, 0.1M TRIS-HCL PH 8.5), drop equilibrated against 1 ml reservoir solution , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, CRYO Reservoir + 20%V Ethylene glycol