3KJM
Leu492Ala mutant of Maize cytokinin oxidase/dehydrogenase complexed with phenylurea inhibitor CPPU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 248.960, 50.460, 50.770 |
| Unit cell angles | 90.00, 93.66, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.21846 |
| Rwork | 0.217 |
| R-free | 0.25518 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qpm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.987 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 | |
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.045 | 0.016 | 0.497 |
| Number of reflections | 49556 | ||
| <I/σ(I)> | 16.46 | 52.8 | 2.1 |
| Completeness [%] | 99.1 | 93.9 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 31% (w/v) PEG 1500, 0.5% (w/v)n-octyl beta-D-glucoside, 0.1 M Tris-HCl, pH 7.0, vapor diffusion, hanging drop, temperature 293K |
