3KJL
Sgf11:Sus1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0719 |
| Spacegroup name | P 61 |
| Unit cell lengths | 68.670, 68.670, 232.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.943 - 2.700 |
| R-factor | 0.217 |
| Rwork | 0.213 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kik |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.623 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.4_129)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.400 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.112 | 0.757 |
| Number of reflections | 17082 | |
| <I/σ(I)> | 18.1 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 22.4 | 22.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | see publication, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
