3KHY
Crystal Structure of a propionate kinase from Francisella tularensis subsp. tularensis SCHU S4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-23 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.079, 87.127, 149.661 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.510 - 1.978 |
R-factor | 0.1553 |
Rwork | 0.155 |
R-free | 0.19330 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.011 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.4_115) |
Refinement software | PHENIX ((phenix.refine: 1.4_115)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.000 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.124 | 0.490 |
Number of reflections | 117233 | |
<I/σ(I)> | 18 | 5.7 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.6 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | NaFormate 4M, 0.3M 195 NDSB, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |