3KHE
Crystal structure of the calcium-loaded calmodulin-like domain of the CDPK, 541.m00134 from toxoplasma gondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .97932 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.838, 101.015, 107.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.950 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.814 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.156 | 0.878 |
| Number of reflections | 37365 | |
| <I/σ(I)> | 3.8 | 2.58 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 24 % PEG3350, 0.1 M NH4SO4, 0.1 M BisTris 6.5, 2 mM CaCl2, 4 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
