3KGX

Crystal structure of Putative aminotransferase (AAH25799.1) from MUS MUSCULUS at 1.80 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-18
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	61.391, 112.565, 117.351
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.696 - 1.800
R-factor	0.175
Rwork	0.173
R-free	0.20700
Structure solution method	MR
Starting model (for MR)	3kgw
RMSD bond length	0.015
RMSD bond angle	1.832
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.696	29.700	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.082	0.035	0.684
Total number of observations		3162	20105
Number of reflections	76087
<I/σ(I)>	10.7	15.2	1.1
Completeness [%]	99.9	97.8	100
Redundancy	3.5	3.3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	20.0000% polyethylene glycol 3350, 0.2000M ammonium fluoride, No Buffer pH 6.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K