3KGT
V30M mutant human transthyretin (TTR) complexed with genistein (V30M:GEN) pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.4586 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.270, 85.070, 64.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.360 - 1.950 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.24100 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.163 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.073 | 0.073 |
| Number of reflections | 17463 | |
| Completeness [%] | 97.7 | |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M CaCl2, 0.1 M HEPES pH 7.5, 28% PEG 400, vapor diffusion, hanging drop, temperature 293K |
