3KG2
AMPA subtype ionotropic glutamate receptor in complex with competitive antagonist ZK 200775
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-18 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.715, 109.848, 161.130 |
| Unit cell angles | 85.32, 84.75, 78.92 |
Refinement procedure
| Resolution | 49.890 - 3.600 |
| R-factor | 0.287 |
| Rwork | 0.286 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 3H5V and 1FTL |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.442 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.111 | 0.981 |
| Number of reflections | 69301 | |
| <I/σ(I)> | 17.2 | 2.08 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.7 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | Under paraffin oil, temperature 277K |
