3KFM
Crystal Structure of the GluA4 Ligand-Binding domain L651V mutant in complex with kainate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-23 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 45.690, 48.445, 62.834 |
| Unit cell angles | 90.00, 106.62, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.200 |
| Rwork | 0.190 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3en3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.281 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 13431 | |
| Completeness [%] | 99.3 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 291 | 18% PEG 8000, 0.1M Na-Acetate, 0.2M Ammonium Sulphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
