3KF9
Crystal structure of the SdCen/skMLCK complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2009-09-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9756 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.750, 67.750, 129.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.600 |
R-factor | 0.20427 |
Rwork | 0.200 |
R-free | 0.29021 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qtx |
RMSD bond length | 0.014 |
RMSD bond angle | 1.451 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.104 | 0.235 |
Number of reflections | 10844 | |
<I/σ(I)> | 10.1 | 6.3 |
Completeness [%] | 95.3 | |
Redundancy | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | MPEG 2000, Nacacodylate 0.1M, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |