3KF3
Structure of fructofuranosidase from Schwanniomyces occidentalis complexed with fructose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.93950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.870, 92.270, 116.280 |
Unit cell angles | 90.00, 104.81, 90.00 |
Refinement procedure
Resolution | 49.620 - 1.900 |
R-factor | 0.222 |
Rwork | 0.220 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kf5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.425 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (CCP4_3.3.9) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 112.420 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.126 | 0.450 |
Number of reflections | 114733 | |
<I/σ(I)> | 4.3 | 1.9 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 7.1 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 20% PEG 6000, 0.2M Cl2Mg, 0.1M Hepes, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |