3KEO
Crystal Structure of a Rex-family transcriptional regulatory protein from Streptococcus agalactiae complexed with NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0379 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.270, 92.410, 47.190 |
| Unit cell angles | 90.00, 91.26, 90.00 |
Refinement procedure
| Resolution | 27.070 - 1.500 |
| R-factor | 0.17845 |
| Rwork | 0.175 |
| R-free | 0.23436 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dt5 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.038 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.100 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.084 | 0.528 |
| Number of reflections | 62064 | |
| <I/σ(I)> | 8.42 | 2.26 |
| Completeness [%] | 98.0 | 93.3 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 6000, 175mM magnesium chloride, 100mM HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
