3KE0
Crystal structure of N370S Glucocerebrosidase at acidic pH.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9765 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.465, 285.064, 92.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.17484 |
| Rwork | 0.172 |
| R-free | 0.22921 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ogs |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.780 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.000 | 2.870 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 37753 | |
| <I/σ(I)> | 7.3 | 2.5 |
| Completeness [%] | 99.5 | 94.1 |
| Redundancy | 6.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 294 | 1M Ammonium Sulfate/0.2M Potassium Chloride/0.05M Guanidine-Hydrochloride/0.1M Sodium Citrate, pH5.4, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
