3KDW

Crystal structure of Putative sugar binding protein (YP_001300177.1) from Bacteroides vulgatus ATCC 8482 at 1.70 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97929
Spacegroup name	P 31 2 1
Unit cell lengths	75.303, 75.303, 87.128
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.490 - 1.700
R-factor	0.154
Rwork	0.152
R-free	0.19100
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.518
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.490	28.490	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.040	0.019	0.550
Number of reflections	31900	6080	5898
<I/σ(I)>	16.69	48.6	2
Completeness [%]	99.5	99.4	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.2000M (NH4)2HPO4, 50.0000% MPD, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K