3KCQ
Crystal structure of phosphoribosylglycinamide formyltransferase from anaplasma phagocytophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-16 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97953 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.450, 125.247, 138.537 |
| Unit cell angles | 90.00, 95.44, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zly |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.366 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.081 | 0.449 |
| Number of reflections | 45778 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 85.4 | 81.9 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | EMERALD JCSG SCREEN CONDITION A2. 100 MM CITRATE PH 5.0, 20% PEG 3000. PROTEIN CONCENTRATION 54 MG/ML, VAPOR DIFFUSIONI, SITTING DROP, TEMPERATURE 293K, VAPOR DIFFUSION, SITTING DROP |
