3KC2
Crystal structure of mitochondrial HAD-like phosphatase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-04 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.734, 67.095, 76.899 |
| Unit cell angles | 90.00, 110.45, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.550 |
| R-factor | 0.145 |
| Rwork | 0.144 |
| R-free | 0.16900 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.509 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.063 | 0.766 |
| Number of reflections | 99129 | |
| <I/σ(I)> | 24.3 | 1.9 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 200mM Na,K phosphate, Hepes, 2mM MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
