3KB1
Crystal Structure of the Nucleotide-binding protein AF_226 in complex with ADP from Archaeoglobus fulgidus, Northeast Structural Genomics Consortium Target GR157
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.440, 67.682, 79.403 |
| Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26500 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX (then SOLVE/RESOLVE) |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.093 | 0.254 |
| Number of reflections | 26385 | |
| <I/σ(I)> | 15 | 3.07 |
| Completeness [%] | 98.3 | 88.1 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 100 mM NaAcetate (pH 4.6), 30% MPD, and 200 mM NaCl. , VAPOR DIFFUSION, SITTING DROP, temperature 291K |
